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anti integrin α3β1  (Bioss)


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    Structured Review

    Bioss anti integrin α3β1
    Anti Integrin α3β1, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+integrin+%CE%B13%CE%B21/Integrin+Alpha+3+%2B+Beta+1+Antibody/pm41699655-207-17-19
    Average 93 stars, based on 12 article reviews
    anti integrin α3β1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Control:

    Article Title: Polyphenolic nanodot-integrated spindle nanoplatform heals oral mucositis via microbiota restoration and glycolysis inhibition.
    Article Snippet: The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.. The following primary antibodies were used: anti-GADPH (Proteintech, 60004-1-Ig; dilution, 1:10000), anti-Integrin β1 (CST, 4706S; dilution, 1:1000), anti-Integrin α3β1 (Bioss, bs1057R-50 μl; dilution, 1:1000), anti-Galectin 3 (Thermo, 14-5301-81; dilution,. AR TIC LE I PR ES S ARTICLE IN PRESS 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).AR TIC LE I PR ES S ARTICLE IN PRESS 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).

    Article Title: Polyphenolic nanodot-integrated spindle nanoplatform heals oral mucositis via microbiota restoration and glycolysis inhibition
    Article Snippet: The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.. The following primary antibodies were used: anti-GADPH (Proteintech, 60004-1-Ig; dilution, 1:10000), anti-Integrin β1 (CST, 4706 S; dilution, 1:1000), anti-Integrin α3β1 (Bioss, bs-1057R-50 μl; dilution, 1:1000), anti-Galectin 3 (Thermo, 14–5301-81; dilution, 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).. Then, secondary antibodies were added, and the membrane was incubated for 1 h at room temperature.Then, secondary antibodies were added, and the membrane was incubated for 1 h at room temperature.

    Immunofluorescence:

    Article Title: Polyphenolic nanodot-integrated spindle nanoplatform heals oral mucositis via microbiota restoration and glycolysis inhibition.
    Article Snippet: The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.. The following primary antibodies were used: anti-GADPH (Proteintech, 60004-1-Ig; dilution, 1:10000), anti-Integrin β1 (CST, 4706S; dilution, 1:1000), anti-Integrin α3β1 (Bioss, bs1057R-50 μl; dilution, 1:1000), anti-Galectin 3 (Thermo, 14-5301-81; dilution,. AR TIC LE I PR ES S ARTICLE IN PRESS 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).AR TIC LE I PR ES S ARTICLE IN PRESS 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).

    Article Title: Polyphenolic nanodot-integrated spindle nanoplatform heals oral mucositis via microbiota restoration and glycolysis inhibition
    Article Snippet: The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.. The following primary antibodies were used: anti-GADPH (Proteintech, 60004-1-Ig; dilution, 1:10000), anti-Integrin β1 (CST, 4706 S; dilution, 1:1000), anti-Integrin α3β1 (Bioss, bs-1057R-50 μl; dilution, 1:1000), anti-Galectin 3 (Thermo, 14–5301-81; dilution, 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).. Then, secondary antibodies were added, and the membrane was incubated for 1 h at room temperature.Then, secondary antibodies were added, and the membrane was incubated for 1 h at room temperature.

    Staining:

    Article Title: Polyphenolic nanodot-integrated spindle nanoplatform heals oral mucositis via microbiota restoration and glycolysis inhibition.
    Article Snippet: The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.. The following primary antibodies were used: anti-GADPH (Proteintech, 60004-1-Ig; dilution, 1:10000), anti-Integrin β1 (CST, 4706S; dilution, 1:1000), anti-Integrin α3β1 (Bioss, bs1057R-50 μl; dilution, 1:1000), anti-Galectin 3 (Thermo, 14-5301-81; dilution,. AR TIC LE I PR ES S ARTICLE IN PRESS 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).AR TIC LE I PR ES S ARTICLE IN PRESS 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).

    Article Title: Polyphenolic nanodot-integrated spindle nanoplatform heals oral mucositis via microbiota restoration and glycolysis inhibition
    Article Snippet: The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.The membrane was blocked with 5% skim milk for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C.. The following primary antibodies were used: anti-GADPH (Proteintech, 60004-1-Ig; dilution, 1:10000), anti-Integrin β1 (CST, 4706 S; dilution, 1:1000), anti-Integrin α3β1 (Bioss, bs-1057R-50 μl; dilution, 1:1000), anti-Galectin 3 (Thermo, 14–5301-81; dilution, 1:1000), anti-FAK (Bioss, bs-20735R-50 μl; dilution, 1:1000), and anti-RAC1 (Finetest, FNab07064-50 μg; dilution, 1:1000).. Then, secondary antibodies were added, and the membrane was incubated for 1 h at room temperature.Then, secondary antibodies were added, and the membrane was incubated for 1 h at room temperature.



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    Image Search Results


    Comparison of α3β1 integrin protein levels in rat endometrium at the time of embryo implantation. All values were presented as mean±SEM. Error bars represent standard error of mean. p<0.05 was considered statistically significant. Data analysis reveals that significant differences were observed between all four groups. In western blot image, D=diabetic control, C=control group, D+P=diabetic rats treated with pioglitazone and D+M=diabetic rats treated with metformin

    Journal: Journal of Reproduction & Infertility

    Article Title: A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation

    doi:

    Figure Lengend Snippet: Comparison of α3β1 integrin protein levels in rat endometrium at the time of embryo implantation. All values were presented as mean±SEM. Error bars represent standard error of mean. p<0.05 was considered statistically significant. Data analysis reveals that significant differences were observed between all four groups. In western blot image, D=diabetic control, C=control group, D+P=diabetic rats treated with pioglitazone and D+M=diabetic rats treated with metformin

    Article Snippet: The membranes were blotted with rabbit anti- OPN (cat. no. ab8448; Abcam) and rabbit anti α3β1 integrin (cat no. bs-1057R, bioss) primary antibodies at a dilution of 1:250 and incubated overnight at 4° C . Following incubation, the membranes were washed three times with phosphate-buffered saline containing 0.05% Tween 20 and then incubated with mouse anti-rabbit IgG – HRP secondary antibody (cat. nos.

    Techniques: Western Blot

    CoMtb-driven matrix metalloproteinase (MMP)-1 gene expression and secretion is regulated by integrin α2β1 and actin cytoskeleton. Human bronchial epithelial cells (HBECs) were seeded in matrix type I collagen (mColl-I) or wells coated with anti-integrin antibodies and stimulated with CoMtb or control medium. (A) MMP-1 secretion from HBECs in the presence or absence of coated anti-integrin α2β1 or anti-α3β1 antibodies. (B) Fold-change in MMP-1 mRNA accumulation in the presence or absence of coated anti-integrin α2β1 or anti-α3β1 antibodies. MMP-1 mRNA copies were normalized to 18S mRNA and fold-change is relative to mean MMP-1 expression in control wells without extracellular matrix. (C) MMP-1 from HBECs pre-incubated for 2 h with 4 µg/ml cytochalasin D before stimulation with CoMtb for 24 h. This figure shows fold-change relative to mean MMP-1 secretion by the respective controls in the absence of CoMtb. Plots show mean and SD of assays performed in triplicate, which were replicated on three occasions. p -Values were obtained with one-way ANOVA with Tukey’s range test. *** p < 0.001; **** p < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Integrin α2β1 Expression Regulates Matrix Metalloproteinase-1-Dependent Bronchial Epithelial Repair in Pulmonary Tuberculosis

    doi: 10.3389/fimmu.2018.01348

    Figure Lengend Snippet: CoMtb-driven matrix metalloproteinase (MMP)-1 gene expression and secretion is regulated by integrin α2β1 and actin cytoskeleton. Human bronchial epithelial cells (HBECs) were seeded in matrix type I collagen (mColl-I) or wells coated with anti-integrin antibodies and stimulated with CoMtb or control medium. (A) MMP-1 secretion from HBECs in the presence or absence of coated anti-integrin α2β1 or anti-α3β1 antibodies. (B) Fold-change in MMP-1 mRNA accumulation in the presence or absence of coated anti-integrin α2β1 or anti-α3β1 antibodies. MMP-1 mRNA copies were normalized to 18S mRNA and fold-change is relative to mean MMP-1 expression in control wells without extracellular matrix. (C) MMP-1 from HBECs pre-incubated for 2 h with 4 µg/ml cytochalasin D before stimulation with CoMtb for 24 h. This figure shows fold-change relative to mean MMP-1 secretion by the respective controls in the absence of CoMtb. Plots show mean and SD of assays performed in triplicate, which were replicated on three occasions. p -Values were obtained with one-way ANOVA with Tukey’s range test. *** p < 0.001; **** p < 0.001.

    Article Snippet: Therefore, to selectively analyze engagement of specific integrins, plates were coated with goat anti-mouse IgG monoclonal antibody overnight at 4°C, washed with PBS, and coated for 2 h at room temperature with either 20 μg/ml of anti-integrin α2β1 (clone BHA2.1) antibody or anti-integrin α3β1 (clone M-KID2) antibody, both from Millipore (Hertfordshire, UK).

    Techniques: Gene Expression, Control, Expressing, Incubation